| | Pop | R&B | Date | Label | Artist | Song | Composer |
|---|
| | 11 | 5 | Oct-1965 | Atlantic | Barbara Lewis | Make Me Your Baby | Atkins-Miller |
| | 11 | 5 | Jul-1965 | Atlantic | Barbara Lewis | Baby, I'm Yours | Van McCoy |
| | 25 | 12 | Feb-1965 | Motown/Tamla | Brenda Holloway | When I'm Gone | William Robinson Jr. |
| | | | May-1965 | Chess | Buddy Guy | Out of Sight | Ted Wright |
| | | | May-1965 | Testament | Dr. Isaiah Ross | Feel So Good | Isaiah Ross |
| | 33 | 1 | Oct-1965 | Checker | Fontella Bass | Rescue Me | Raynard Miner, C. Smith |
| | 1 | 1 | Apr-1965 | Motown | Four Tops | I Can't Help Myself (Sugar Pie Honey Bunch) | Holland-Dozier-Holland |
| | 5 | 2 | Jul-1965 | Motown | Four Tops | It's The Same Old Song | Holland-Dozier-Holland |
| | 24 | 9 | Jan-1965 | Motown | Four Tops | Ask The Lonely | Stevenson-Hunter |
| | 3 | 1 | Nov-1965 | King | James Brown | I Got You (I Feel Good) | James Brown |
| | 8 | 1 | Aug-1965 | King | James Brown | Papa's Got A Brand New Bag | James Brown |
| | 23 | 1 | Oct-1965 | Dial | Joe Tex | I Want to (Do everything for you) | Joe Tex |
| | 88 | 4 | Sep-1965 | JoDa | Johnny Nash | Let's Move & Groove Together | Unknown |
| | 4 | 1 | Jan-1965 | Motown/Soul | Jr. Walker & The All Stars | Shotgun | Autry DeWalt |
| | 50 | 4 | Sep-1965 | Motown/Gordy | Kim Weston | Take Me In Your Arms | Holland-Dozier-Holland |
| | 10 | 5 | Jul-1965 | DCP | Little Anthony & the Imperials | Hurt So Bad | Hart, Randazzo, Wilding |
| | 25 | 1 | May-1965 | Checker | Little Milton | We're Gonna Make It | Barge, Davis, Miner, Smith |
| | 8 | 5 | Feb-1965 | Motown/Gordy | Martha & The Vandellas | Nowhere To Run | Holland-Dozier-Holland |
| | 8 | 1 | Nov-1965 | Motown | Marvin Gaye | Ain't That Peculiar | Moore, Robinson, Rogers, Tarplin |
| | 8 | 1 | May-1965 | Motown | Marvin Gaye | I'll be Doggone | Moore, Robinson, Tarplin |
| | 21 | 2 | Jun-1965 | Volt | Otis Redding | I've Been Loving You Too Long (To Stop Now) | Redding-Butler |
| | 41 | 10 | Feb-1965 | Volt | Otis Redding | Mr Pitiful | Steve Cropper, Otis Redding |
| | 35 | 4 | Sep-1965 | Volt | Otis Redding | Respect | Otis Redding |
| | 90 | 7 | Nov-1965 | Atlantic | Sam & Dave | You Don't Know Like I Know | Hayes-Porter |
| | 7 | 3 | Jan-1965 | RCA | Sam Cooke | Shake | Sam Cooke |
| | 22 | 1 | Apr-1965 | Atlantic | Solomon Burke | Got to Get You Off My Mind | Burke, Burke, Moore |
| | | | Apr-1965 | Paramount | Son House | Death Letter | Son House |
| | 3 | 1 | Jul-1965 | Motown/Tamla | Stevie Wonder | Uptight (Everything's Alright) | Cosby-Moy-Wonder |
| | 72 | 3 | Dec-1965 | Motown/VIP | The Elgins | Darling Baby | Holland-Dozier-Holland |
| | 7 | 3 | Nov-1965 | Motown/Tamla | The Marvelettes | Don't Mess With Bill | William Robinson Jr. |
| | 16 | 2 | Jun-1965 | Motown/Tamla | The Miracles | The Tracks of My Tears | Robinson-Moore |
| | 16 | 4 | Mar-1965 | Motown/Tamla | The Miracles | Ooo Baby Baby | Robinson-Moore |
| | 1 | 2 | Feb-1965 | Motown | The Supremes | Stop In the Name of Love | Holland-Dozier-Holland |
| | 1 | 1 | May-1965 | Motown | The Supremes | Back in My Arms Again | Holland-Dozier-Holland |
| | 11 | 6 | Aug-1965 | Motown | The Supremes | Nothing But Heartache | Holland-Dozier-Holland |
| | 1 | 2 | Oct-1965 | Motown | The Supremes | I Hear A Symphony | Holland-Dozier-Holland |
| | 21 | 1 | Aug-1965 | Atlantic | Wilson Pickett | In the Midnight Hour | Pickett-Cropper |
